| Gene | 2410022L05Rik |
| Chromosome | 8 |
| Deletion Start | 13,890,232 |
| Deletion End | 13,884,940 |
| Deletion Size | 5,293 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,889,618 | 13,889,638 | CTACTGCGAGCTTCTTGACTG |
| TUP | 13,889,591 | 13,889,614 | ACATCCTGTTTCCCAGAAGCCGCA |
| TUR | 13,889,539 | 13,889,563 | GCTCTGGTAAGCGTTCTATTCATTC |
| TDF | 13,885,161 | 13,885,184 | GGGAGCATTTCCCAAGAGATCAAG |
| TDP | 13,885,138 | 13,885,159 | CTTGGGTGTGCTGTGCACCACA |
| TDR | 13,885,097 | 13,885,116 | GAGGTGGATGGTGCCAACTG |
PCR Primers
| Primer | Sequence |
| SU | GTCATTTCACAATGCAGTAGG |
| SD | ACAAGCCACACAGTCACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14029A-A4 | VGB6 | C57BL/6NTac | |
| 14029A-C8 | VGB6 | C57BL/6NTac | |
| 14029A-D9 | VGB6 | C57BL/6NTac | |
