| Gene | Prr5l |
| Chromosome | 2 |
| Deletion Start | 101,772,290 |
| Deletion End | 101,746,731 |
| Deletion Size | 25,560 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,772,211 | 101,772,232 | GGCCTAGACCACGCTTTATGAG |
| TUP | 101,772,186 | 101,772,204 | TGTGCTCAGCGAGCTGCCC |
| TUR | 101,772,111 | 101,772,129 | TGGTCCCACAAGCCCTTAC |
| TDF | 101,747,395 | 101,747,414 | TGCTGGAAGACCAGAGTCAC |
| TDP | 101,747,370 | 101,747,394 | TGAACCACCTACCCAGCTGCTTGAA |
| TDR | 101,747,333 | 101,747,357 | GGCTACAAAGAAAGACCTCCATCTC |
PCR Primers
| Primer | Sequence |
| SU | GGCTAGCTTTCTGCTAGGGC |
| SD | CAGACCAGATGGACCAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14030A-H9 | VGB6 | C57BL/6NTac | |
| 14030C-B2 | VGB6 | C57BL/6NTac | |
