| Gene | 2610019F03Rik |
| Chromosome | 8 |
| Deletion Start | 13,974,639 |
| Deletion End | 13,953,779 |
| Deletion Size | 20,861 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,974,242 | 13,974,260 | GGGTTCGGCTTGAGACACC |
| TUP | 13,974,209 | 13,974,229 | TGACCGCTTCCAGGCCAGGTA |
| TUR | 13,974,161 | 13,974,181 | GGCACCATGATTCAGGACTTC |
| TDF | 13,953,844 | 13,953,861 | TCAGCGGGAAAGCTGGTC |
| TDP | 13,953,819 | 13,953,843 | AGCATCAGGCGACAGAGCAAAGGCC |
| TDR | 13,953,782 | 13,953,803 | TCGGTTCATTCTCCCTCTTCAC |
PCR Primers
| Primer | Sequence |
| SU | TTGGTGCGTCAGTGTTCTTG |
| SD | GGAAGATGCTTCTTGCGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14031A-B3 | VGB6 | C57BL/6NTac | |
| 14031A-B12 | VGB6 | C57BL/6NTac | |
| 14031A-G6 | VGB6 | C57BL/6NTac | |
| 14031A-H3 | VGB6 | C57BL/6NTac | |
| 14031A-E3 | VGB6 | C57BL/6NTac | |
| 14031A-E11 | VGB6 | C57BL/6NTac | |
