| Gene | Atat1 |
| Chromosome | 17 |
| Deletion Start | 35,910,032 |
| Deletion End | 35,900,823 |
| Deletion Size | 9,210 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,909,599 | 35,909,620 | TGAGGAAGAGTTCCAAGCAAAG |
| TUP | 35,909,576 | 35,909,598 | TCAGAGACCCTGTGGAGGAGCCT |
| TUR | 35,909,511 | 35,909,533 | GTCAGACACTCGCTAGGTTACAG |
| TDF | 35,901,359 | 35,901,381 | CGCAGACGCACCAGGTAATAAAG |
| TDP | 35,901,326 | 35,901,354 | CAAGTTTGGGAGCCTCTGGATGTGATGAA |
| TDR | 35,901,282 | 35,901,303 | TTCACGTTGGTTGGAGATTCAC |
PCR Primers
| Primer | Sequence |
| SU | CTTCACCCGTTCTTGACTCC |
| SD | AATCCATTGGCAGGACACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14032A-B5 | VGB6 | C57BL/6NTac | |
| 14032A-D10 | VGB6 | C57BL/6NTac | |
| 14032A-C2 | VGB6 | C57BL/6NTac | |
