| Gene | Fam72a |
| Chromosome | 1 |
| Deletion Start | 131,528,704 |
| Deletion End | 131,539,001 |
| Deletion Size | 10,298 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,528,831 | 131,528,853 | CTACAGACATCCCTCCTACCAAG |
| TUP | 131,528,858 | 131,528,880 | TCTGACGGGTGGCCCTTTAGCTG |
| TUR | 131,528,883 | 131,528,901 | GGACCCATGCCATCCACTG |
| TDF | 131,538,370 | 131,538,391 | GGAGACAGAGTTGGAGCAGATG |
| TDP | 131,538,397 | 131,538,417 | TCCAGAGTTCCTTTTAAGGTC |
| TDR | 131,538,493 | 131,538,511 | GGAAGCGCAGTCTAACCAC |
PCR Primers
| Primer | Sequence |
| SU | CTTTAAAGGGAAGAAGCCCG |
| SD | TGGGTGGCTGATATAGGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 142 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14033A-A3 | VGB6 | C57BL/6NTac | |
| 14033A-B10 | VGB6 | C57BL/6NTac | |
| 14033A-H2 | VGB6 | C57BL/6NTac | |
