| Gene | 2700094K13Rik |
| Chromosome | 2 |
| Deletion Start | 84,670,583 |
| Deletion End | 84,669,959 |
| Deletion Size | 625 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,670,526 | 84,670,545 | TATGGAGACGGTGGACAAGC |
| TUP | 84,670,483 | 84,670,506 | CGCGACCGTGGTCATTGAGCATTG |
| TUR | 84,670,434 | 84,670,453 | CCGCCAGAGCCAACTCTTTC |
| TDF | 84,670,099 | 84,670,120 | CCAGGTGTTGAACTCTGGACTG |
| TDP | 84,670,057 | 84,670,085 | CCCTCCACGAAAGCTCAAATTTCCTGAGC |
| TDR | 84,669,996 | 84,670,020 | AGGACTCTTTCCCAACCTCTTTATG |
PCR Primers
| Primer | Sequence |
| SU | CTTCCCTGAGATTGGTCTGG |
| SD | GGGATGGGTTGAGGCTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14034A-H10 | VGB6 | C57BL/6NTac | |
| 14034A-B4 | VGB6 | C57BL/6NTac | |
| 14034A-G6 | VGB6 | C57BL/6NTac | |
| 14034A-C5 | VGB6 | C57BL/6NTac | |
| 14034A-D4 | VGB6 | C57BL/6NTac | |
