| Gene | 2810021B07Rik |
| Chromosome | 13 |
| Deletion Start | 17,695,488 |
| Deletion End | 17,697,069 |
| Deletion Size | 1,582 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,695,842 | 17,695,862 | TGCCGAACTCTTCCTGTGATG |
| TUP | 17,695,868 | 17,695,896 | CGTGATACGAAACATTCCCTTTCCGTGGA |
| TUR | 17,695,966 | 17,695,988 | TCCGCCACTTATGGTAGTCTTAG |
| TDF | 17,696,388 | 17,696,411 | GCTAACAACTTGTATCTCGGTGGT |
| TDP | 17,696,413 | 17,696,435 | TTGCAAAATTGAATATGCTCACC |
| TDR | 17,696,552 | 17,696,572 | CCAGTACAAGCAGTGGTTCAC |
PCR Primers
| Primer | Sequence |
| SU | GGTTTCCTCTCTTCCGCTTC |
| SD | TTCTATGATACACACTCCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 147 bp | + | + | - |
| TDF | TDR | 185 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14035A-A3 | VGB6 | C57BL/6NTac | |
| 14035A-H4 | VGB6 | C57BL/6NTac | |
| 14035A-C2 | VGB6 | C57BL/6NTac | |
| 14035A-C5 | VGB6 | C57BL/6NTac | |
| 14035A-B7 | VGB6 | C57BL/6NTac | |
| 14035A-G4 | VGB6 | C57BL/6NTac | |
