| Gene | Zfp788 |
| Chromosome | 7 |
| Deletion Start | 41,647,587 |
| Deletion End | 41,650,647 |
| Deletion Size | 3,061 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,648,249 | 41,648,269 | TCTTCACCAAGACACCAACTC |
| TUP | 41,648,270 | 41,648,289 | TGACAAACATCTTCCCTGGT |
| TUR | 41,648,305 | 41,648,323 | GCATCCCGCCACTTTCATC |
| TDF | 41,650,120 | 41,650,142 | TGCTTACTCCAGACCCATGAAAG |
| TDP | 41,650,146 | 41,650,174 | TCACAGTGGTGTGAAACCCTACGAATGTA |
| TDR | 41,650,199 | 41,650,220 | TGGACCCGAAAGGAACTATGAC |
PCR Primers
| Primer | Sequence |
| SU | TGGAAGGACTAGAGATCCATC |
| SD | ATTCACAGAATTCCCTTCTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14036B-C5 | VGB6 | C57BL/6NTac | |
| 14036C-B5 | VGB6 | C57BL/6NTac | |
