| Gene | Spata24 |
| Chromosome | 18 |
| Deletion Start | 35,662,125 |
| Deletion End | 35,656,646 |
| Deletion Size | 5,480 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,661,558 | 35,661,576 | AGTGGGTGCCACGCTAATG |
| TUP | 35,661,529 | 35,661,556 | CGTCATAGTTACATCAGTGCCGGGTCCG |
| TUR | 35,661,508 | 35,661,528 | CACAAAGCCCATGACCTCCAG |
| TDF | 35,656,860 | 35,656,877 | TGTGCAGCCTACGACCAG |
| TDP | 35,656,830 | 35,656,858 | TGGCTCCATTGCAGGAATCACATATCTGA |
| TDR | 35,656,752 | 35,656,773 | CGCATTCCTGTGGCTTTCTTAC |
PCR Primers
| Primer | Sequence |
| SU | TTTGTTGCTGCAGTGTTGGG |
| SD | CAAACCTAACCACCCAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 126 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14044A-H10 | VGB6 | C57BL/6NTac | |
| 14044A-C9 | VGB6 | C57BL/6NTac | |
| 14044A-D4 | VGB6 | C57BL/6NTac | |
| 14044A-E6 | VGB6 | C57BL/6NTac | |
| 14044A-E8 | VGB6 | C57BL/6NTac | |
| 14044A-D11 | VGB6 | C57BL/6NTac | |
