| Gene | 8030411F24Rik |
| Chromosome | 2 |
| Deletion Start | 148,782,111 |
| Deletion End | 148,785,882 |
| Deletion Size | 3,772 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,782,549 | 148,782,569 | CCACCTTTAGCTTCCGCACAG |
| TUP | 148,782,579 | 148,782,607 | TGAAAGCCTAGCTCATCATGTGGGATTGA |
| TUR | 148,782,615 | 148,782,637 | CTCCATCACATCTATTCCACACC |
| TDF | 148,785,279 | 148,785,299 | GGGACCTTGAGGATGGCTTTC |
| TDP | 148,785,300 | 148,785,325 | CATTGCGGGACCCTGTTAGGGTTTGT |
| TDR | 148,785,383 | 148,785,403 | CCAGGCACTTCCCAACAGAAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCCAGGAATGAGTTCACC |
| SD | CAGCTTGTGGGCTTCTAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14051A-A2 | VGB6 | C57BL/6NTac | |
| 14051A-F11 | VGB6 | C57BL/6NTac | |
| 14051A-A10 | VGB6 | C57BL/6NTac | |
| 14051A-F3 | VGB6 | C57BL/6NTac | |
| 14051A-C12 | VGB6 | C57BL/6NTac | |
| 14051A-C1 | VGB6 | C57BL/6NTac | |
