| Gene | 9530077C05Rik |
| Chromosome | 9 |
| Deletion Start | 22,411,728 |
| Deletion End | 22,444,241 |
| Deletion Size | 32,514 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,411,737 | 22,411,756 | ATTCGCGTAACGGGTGAGTG |
| TUP | 22,411,757 | 22,411,778 | TAGGCACTGCTGGCCCAAGTGG |
| TUR | 22,411,813 | 22,411,831 | GCACCTGGTCTTCCGAGTC |
| TDF | 22,444,078 | 22,444,102 | TACGAAGATGTGGATCGCTTAAAAG |
| TDP | 22,444,106 | 22,444,133 | TGGCAGTTACTGAAAACATGCGAGTCCC |
| TDR | 22,444,150 | 22,444,168 | GCTCCATGTACCGGCTGTG |
PCR Primers
| Primer | Sequence |
| SU | AAGGCAGGATCGTCTCCTAG |
| SD | TAGACTGGCCACCTCCAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14053A-B5 | VGB6 | C57BL/6NTac | |
| 14053A-G5 | VGB6 | C57BL/6NTac | |
| 14053A-E7 | VGB6 | C57BL/6NTac | |
| 14053A-E4 | VGB6 | C57BL/6NTac | |
| 14053A-F10 | VGB6 | C57BL/6NTac | |
| 14053A-E2 | VGB6 | C57BL/6NTac | |
