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Sequence Downloads and Strategy Information
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,123,122 | 104,123,142 | GGCCTGGCTTCTATTCCCTAC |
| TUP | 104,123,144 | 104,123,168 | AGCCTCTGCGCTTCTTCTACTCGCC |
| TUR | 104,123,182 | 104,123,201 | AGGGCCGAAGTTGCCATCTC |
| TDF | 104,124,340 | 104,124,361 | TTGCTTCTCAGGGTGTAGATGG |
| TDP | 104,124,363 | 104,124,389 | ACATGCCTGGAATCCCAGTACTTAGGA |
| TDR | 104,124,410 | 104,124,427 | CAGGTGGCTCTGGAACTC |
PCR Primers
| Primer | Sequence |
| SU | GAAGGCTTCTGGGAGGAGTC |
| SD | AGAGCCAACATCAGGACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the KOMP Repository.| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14058A-G4 | VGB6 | C57BL/6NTac | |
| 14058B-B4 | VGB6 | C57BL/6NTac |
