| Gene | BC005537 |
| Chromosome | 13 |
| Deletion Start | 24,803,384 |
| Deletion End | 24,812,788 |
| Deletion Size | 9,405 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,804,113 | 24,804,136 | TGAAAGTGGTCTGCCTCTAATGAG |
| TUP | 24,804,140 | 24,804,163 | TGTAATCTTAGCATGCAATCTGTT |
| TUR | 24,804,175 | 24,804,197 | GGGCAACAAGCATGGAAAGTTAC |
| TDF | 24,812,670 | 24,812,688 | TGCAGCATCTGCCTCTACC |
| TDP | 24,812,691 | 24,812,714 | CCACAGGAACAGCTCACCCACTGG |
| TDR | 24,812,716 | 24,812,738 | GTGATCCTCTATGGTGCCAACTG |
PCR Primers
| Primer | Sequence |
| SU | TACTGCGCACCTAAATCCTG |
| SD | CCAGCACAAACATGAAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14060A-F9 | VGB6 | C57BL/6NTac | |
| 14060A-B1 | VGB6 | C57BL/6NTac | |
| 14060A-B2 | VGB6 | C57BL/6NTac | |
| 14060A-F8 | VGB6 | C57BL/6NTac | |
| 14060A-C11 | VGB6 | C57BL/6NTac | |
