| Gene | Zfp940 |
| Chromosome | 7 |
| Deletion Start | 29,850,947 |
| Deletion End | 29,844,703 |
| Deletion Size | 6,245 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,850,838 | 29,850,862 | CAACTGGTAAGGGATGGTTGTGAAG |
| TUP | 29,850,813 | 29,850,835 | CTCTACAGCTGGCGGCCATTGCT |
| TUR | 29,850,782 | 29,850,804 | TTGGTGCTTTGTCAGATGGTTAG |
| TDF | 29,844,890 | 29,844,914 | GGTCAAAGAGCACATCTTACCAAAC |
| TDP | 29,844,858 | 29,844,887 | CAGAGAATTCACACTGGTGAAGAAGGCCCT |
| TDR | 29,844,836 | 29,844,857 | TTTCCACGCTCTGACATTCATC |
PCR Primers
| Primer | Sequence |
| SU | TTCAAAGAAGCCTTAACCTTTC |
| SD | TGCAAAGGATGTGGTGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14061A-H2 | VGB6 | C57BL/6NTac | |
| 14061C-F9 | VGB6 | C57BL/6NTac | |
| 14061C-F10 | VGB6 | C57BL/6NTac | |
