| Gene | BC048355 |
| Chromosome | 17 |
| Deletion Start | 46,496,813 |
| Deletion End | 46,499,245 |
| Deletion Size | 2,433 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,497,025 | 46,497,045 | GACCGGCTGACTAGGGACTTG |
| TUP | 46,497,046 | 46,497,071 | CCGGATAGGAGACCCACTGGCTAGTA |
| TUR | 46,497,087 | 46,497,105 | TCACATGAGCTGCGGGTTC |
| TDF | 46,498,715 | 46,498,734 | AGCCGATCCTGTGCCTGTTC |
| TDP | 46,498,735 | 46,498,755 | CGACCACAGTCTGGCCGAGGT |
| TDR | 46,498,792 | 46,498,813 | GCCCACTGGGAAAGGAAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGAGAGCCAATCACAGTTGC |
| SD | ACTGTGCCTCCTGAGACGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14062A-B5 | VGB6 | C57BL/6NTac | |
| 14062A-C3 | VGB6 | C57BL/6NTac | |
| 14062A-C4 | VGB6 | C57BL/6NTac | |
| 14062A-D11 | VGB6 | C57BL/6NTac | |
