| Gene | Zfp599 |
| Chromosome | 9 |
| Deletion Start | 22,259,756 |
| Deletion End | 22,247,034 |
| Deletion Size | 12,723 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,259,438 | 22,259,457 | CTGGTGCCTAGACGATCCTG |
| TUP | 22,259,416 | 22,259,437 | TCCCTGGGACCTCGGGCTCTAT |
| TUR | 22,259,366 | 22,259,387 | TCCGAGTTCATGCCACAGATAG |
| TDF | 22,247,392 | 22,247,416 | GATGTAGGTCCCATTGAGGAGTAAG |
| TDP | 22,247,363 | 22,247,388 | TGCATCACCACAAAGCCTGTACGGTG |
| TDR | 22,247,325 | 22,247,347 | CGAATGTTTGTGTCTGCGAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GCCCTGCCTTACAATACAGC |
| SD | ACTTAGAGAGCACTTTTGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14064A-H11 | VGB6 | C57BL/6NTac | |
| 14064A-C10 | VGB6 | C57BL/6NTac | |
| 14064A-D3 | VGB6 | C57BL/6NTac | |
| 14064A-B11 | VGB6 | C57BL/6NTac | |
| 14064A-A7 | VGB6 | C57BL/6NTac | |
