| Gene | Cyr61 |
| Chromosome | 3 |
| Deletion Start | 145,649,789 |
| Deletion End | 145,647,663 |
| Deletion Size | 2,127 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 145,649,283 | 145,649,301 | AACTCAGCCCTGCGACCAC |
| TUP | 145,649,252 | 145,649,273 | TTGGAATGCAATTTCGGCGCCA |
| TUR | 145,649,202 | 145,649,222 | CCAAACCACAAGCCTCTTACC |
| TDF | 145,648,297 | 145,648,315 | GCTGCGGAACTGGCATCTC |
| TDP | 145,648,266 | 145,648,294 | CACGAGTTACCAATGACAACCCAGAGTGC |
| TDR | 145,648,221 | 145,648,240 | TCCACAAGGACGCACTTCAC |
PCR Primers
| Primer | Sequence |
| SU | GCCAACCAACATTCCTGAG |
| SD | ACAAGCAGGGCTTTCACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14067A-A6 | VGB6 | C57BL/6NTac | |
| 14067A-E8 | VGB6 | C57BL/6NTac | |
