| Gene | Fcgrt |
| Chromosome | 7 |
| Deletion Start | 45,103,066 |
| Deletion End | 45,093,199 |
| Deletion Size | 9,868 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,102,148 | 45,102,166 | CCTGCTGGGCTGTGAACTG |
| TUP | 45,102,120 | 45,102,146 | CCTCGGATAATTCCTCAGTGCCCACGG |
| TUR | 45,102,097 | 45,102,117 | CTCACCATTGAGGGCAAACAC |
| TDF | 45,093,854 | 45,093,875 | CCTGTGGTTGGAATCGTTCTTG |
| TDP | 45,093,826 | 45,093,850 | TATTGCTGGTGGTAGTGGCCATCGC |
| TDR | 45,093,770 | 45,093,790 | TCCTGCATATACCTGGCAGAC |
PCR Primers
| Primer | Sequence |
| SU | GCTCCTGAGGCTGAAATCTG |
| SD | CAATGCCTGGTACAGTGTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14071D-A6 | VGB6 | C57BL/6NTac | |
| 14071D-D7 | VGB6 | C57BL/6NTac | |
| 14071D-D2 | VGB6 | C57BL/6NTac | |
| 14071E-H9 | VGB6 | C57BL/6NTac | |
| 14071E-G1 | VGB6 | C57BL/6NTac | |
| 14071E-D1 | VGB6 | C57BL/6NTac | |
