| Gene | Foxo4 |
| Chromosome | X |
| Deletion Start | 101,254,824 |
| Deletion End | 101,259,608 |
| Deletion Size | 4,785 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,255,237 | 101,255,257 | GGACAAGGGTGACAGCAACAG |
| TUP | 101,255,259 | 101,255,287 | TCGGCAGGATGGAAGGTAAGTATGACTCC |
| TUR | 101,255,293 | 101,255,312 | CAAGGGTGGATGGCGTGAAG |
| TDF | 101,259,082 | 101,259,099 | TGTGCTCGCATCTCCTAC |
| TDP | 101,259,100 | 101,259,126 | TGAAGATTCCAGCCATGACAGAATGCC |
| TDR | 101,259,161 | 101,259,181 | GTTATCCATGTCGCACTCCAG |
PCR Primers
| Primer | Sequence |
| SU | AATTTGGGAGGAGTGTCCG |
| SD | AGGCAGGGTAATAAGGCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14072A-A4 | VGB6 | C57BL/6NTac | |
| 14072A-C11 | VGB6 | C57BL/6NTac | |
| 14072A-G7 | VGB6 | C57BL/6NTac | |
