| Gene | Gpr98 |
| Chromosome | 13 |
| Deletion Start | 81,595,287 |
| Deletion End | 81,566,268 |
| Deletion Size | 29,020 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,594,482 | 81,594,504 | GCTTCTTCTCTGTCACTGTCATC |
| TUP | 81,594,458 | 81,594,481 | TCTTACTGAGCCTGCCACTGACGG |
| TUR | 81,594,404 | 81,594,424 | GGGTGTCGTGGTACAAAGGTC |
| TDF | 81,566,844 | 81,566,867 | TCAGAAACGCCAGAAGAAATGAAG |
| TDP | 81,566,817 | 81,566,843 | TCAGTCAGATGGAGAGCACACTACAGC |
| TDR | 81,566,793 | 81,566,811 | GTGGTTCCTCAGCCGTAAC |
PCR Primers
| Primer | Sequence |
| SU | ATCTTGATGAAGAGGATAAATGC |
| SD | AATTGGTTACTGACCAGTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14074D-D3 | VGB6 | C57BL/6NTac | |
| 14074D-D5 | VGB6 | C57BL/6NTac | |
| 14074D-E11 | VGB6 | C57BL/6NTac | |
| 14074D-F12 | VGB6 | C57BL/6NTac | |
| 14074D-H1 | VGB6 | C57BL/6NTac | |
| 14074D-C12 | VGB6 | C57BL/6NTac | |
