| Gene | Hmcn1 |
| Chromosome | 1 |
| Deletion Start | 150,993,048 |
| Deletion End | 150,992,788 |
| Deletion Size | 261 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 150,993,013 | 150,993,034 | GGTCCACACAGTATTCCTGGTG |
| TUP | 150,992,979 | 150,993,004 | TCGTTCTTCCCTAGCTGGAGATGGGA |
| TUR | 150,992,947 | 150,992,967 | AATCTCCTCAGCCCTGCTCTC |
| TDF | 150,992,889 | 150,992,911 | TGTGACTGGGTCCATGTATGATG |
| TDP | 150,992,862 | 150,992,885 | TAGTTCAGGTTATTGAAGGGGCTT |
| TDR | 150,992,794 | 150,992,815 | GAAGGGCACCAAAGCAAAGTTG |
PCR Primers
| Primer | Sequence |
| SU | TGGTCTGCATCTCATTCTCC |
| SD | CTCTGAGCTTGCTGCTGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 118 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14075A-G4 | VGB6 | C57BL/6NTac | |
| 14075A-E9 | VGB6 | C57BL/6NTac | |
| 14075A-H10 | VGB6 | C57BL/6NTac | |
