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Sequence Downloads and Strategy Information
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | GAGACTCTTGAGAGCGGGAAC | ||
| TUP | CCGAAAGACAAACCCGGGAGGGA | ||
| TUR | ACCCTGGTGGCAAGGTATC | ||
| TDF | TCGACCTGTAAGCTGTAGCAAATG | ||
| TDP | CCTTTAATCCTGGCAGGCAGAGGCA | ||
| TDR | GACCAGGCTGACCTCAAAC |
PCR Primers
| Primer | Sequence |
| SU | GCAAAGTCCTAACCAGGGAAG |
| SD | GCCATTCCAGGTATTAGGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the KOMP Repository.| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14076A-H10 | VGB6 | C57BL/6NTac | |
| 14076A-G5 | VGB6 | C57BL/6NTac | |
| 14076A-H6 | VGB6 | C57BL/6NTac | |
| 14076A-H9 | VGB6 | C57BL/6NTac | |
| 14076A-E12 | VGB6 | C57BL/6NTac | |
| 14076A-D9 | VGB6 | C57BL/6NTac |
