Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

14079 Kazald1 Status: ES cell colonies screened / QC positives

Gene	Kazald1
Chromosome	19
Deletion Start	45,076,685
Deletion End	45,079,027
Deletion Size	2,343
Genome Build	38

MGI ID	MGI:2147606
NCBI Gene ID	107250
BAC ID	446H8
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 19

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	45,077,372	45,077,392	GCCAGGTTTGAGGTGCCTAAG
TUP	45,077,393	45,077,417	AGCTCTATCCAGGCAGGTCCTTGCA
TUR	45,077,435	45,077,458	CAGCTATTGGCTATGTGCCTGTTC
TDF	45,078,611	45,078,632	AGCTTTGCGCCCTAGTGATGAG
TDP	45,078,636	45,078,658	ACCTACCGTTGCCTTGCCCGGAA
TDR	45,078,679	45,078,700	AGCACTGTGAGGGTTGCAGAAG

PCR Primers

Primer	Sequence
SU	GGTGGTTTCTGGAGGTGATG
SD	GCCAGAGTCTGTTATGTGCG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	87 bp	+	+	-
TDF	TDR	90 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
14079A-C11	VGB6	C57BL/6NTac
14079A-D2	VGB6	C57BL/6NTac
14079A-C12	VGB6	C57BL/6NTac