| Gene | Sned1 |
| Chromosome | 1 |
| Deletion Start | 93,235,992 |
| Deletion End | 93,265,388 |
| Deletion Size | 29,397 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,236,836 | 93,236,857 | TCCCAAGGGTAATTCCTCACTC |
| TUP | 93,236,862 | 93,236,887 | CCCAGACACGAATGCTGGAGAGGTCT |
| TUR | 93,236,900 | 93,236,917 | AGGCTGGCACTCAGGTTC |
| TDF | 93,264,585 | 93,264,604 | CCTCACTGCACCAGCAGAAC |
| TDP | 93,264,605 | 93,264,634 | CAGGTGTGGAAGCTACTATGGAGACTCAGA |
| TDR | 93,264,652 | 93,264,670 | CCCTGGGCTTTGGCAGATG |
PCR Primers
| Primer | Sequence |
| SU | TCCTCACCTCAACATAGCCC |
| SD | ATTTGTGTGCCAGGAAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14082A-E1 | VGB6 | C57BL/6NTac | |
| 14082A-B10 | VGB6 | C57BL/6NTac | |
| 14082A-C12 | VGB6 | C57BL/6NTac | |
| 14082A-C1 | VGB6 | C57BL/6NTac | |
| 14082A-A10 | VGB6 | C57BL/6NTac | |
| 14082A-F6 | VGB6 | C57BL/6NTac | |
