| Gene | Mif |
| Chromosome | 10 |
| Deletion Start | 75,860,165 |
| Deletion End | 75,859,438 |
| Deletion Size | 728 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,859,974 | 75,859,992 | GCTGGAAGCCAAGGTGTGC |
| TUP | 75,859,942 | 75,859,963 | CGGCTGGAGCTCTCCGGAAGAC |
| TUR | 75,859,907 | 75,859,927 | TTAGACCGCCTGAAAGACTGC |
| TDF | 75,859,626 | 75,859,645 | GGAGGTCAGCAGGCAAAGAG |
| TDP | 75,859,596 | 75,859,618 | CGTTCAGAGGACACTGGCACGCA |
| TDR | 75,859,546 | 75,859,567 | GAGAAAATGAAAGCCTGGCTCA |
PCR Primers
| Primer | Sequence |
| SU | TCTACCTGGAATGCCTCGAC |
| SD | CAGGTGAATAAGTCGGCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14083A-C7 | VGB6 | C57BL/6NTac | |
| 14083A-D11 | VGB6 | C57BL/6NTac | |
| 14083A-H11 | VGB6 | C57BL/6NTac | |
| 14083A-F7 | VGB6 | C57BL/6NTac | |
| 14083A-G8 | VGB6 | C57BL/6NTac | |
| 14083A-H5 | VGB6 | C57BL/6NTac | |
