Gene | Mknk2 |
Chromosome | 10 |
Deletion Start | 80,671,832 |
Deletion End | 80,667,062 |
Deletion Size | 4,771 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 80,671,350 | 80,671,371 | AAGCCCTAGCGTGGCTATCAAG |
TUP | 80,671,318 | 80,671,346 | TGGAAACTTATGTCAGAGACCATGCTGGC |
TUR | 80,671,268 | 80,671,289 | GTTAGGCCAGTCACCCTTAGAG |
TDF | 80,667,618 | 80,667,638 | CTACCGACACCCTTGGTTCTG |
TDP | 80,667,586 | 80,667,611 | TGAGGCCTGGGACCTTAGGAAATGAA |
TDR | 80,667,530 | 80,667,547 | TGCAGGGCTGTGGCTTAG |
PCR Primers
Primer | Sequence |
SU | CTTGGTCTCAGCACTGTGAC |
SD | TGTCCCTGACAACACTGAGG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 104 bp | + | + | - |
TDF | TDR | 109 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
14084A-A9 | VGB6 | C57BL/6NTac | |
14084A-B9 | VGB6 | C57BL/6NTac | |
14084A-D1 | VGB6 | C57BL/6NTac | |
14084A-D2 | VGB6 | C57BL/6NTac | |
14084A-D6 | VGB6 | C57BL/6NTac | |