| Gene | Mknk2 |
| Chromosome | 10 |
| Deletion Start | 80,671,832 |
| Deletion End | 80,667,062 |
| Deletion Size | 4,771 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,671,350 | 80,671,371 | AAGCCCTAGCGTGGCTATCAAG |
| TUP | 80,671,318 | 80,671,346 | TGGAAACTTATGTCAGAGACCATGCTGGC |
| TUR | 80,671,268 | 80,671,289 | GTTAGGCCAGTCACCCTTAGAG |
| TDF | 80,667,618 | 80,667,638 | CTACCGACACCCTTGGTTCTG |
| TDP | 80,667,586 | 80,667,611 | TGAGGCCTGGGACCTTAGGAAATGAA |
| TDR | 80,667,530 | 80,667,547 | TGCAGGGCTGTGGCTTAG |
PCR Primers
| Primer | Sequence |
| SU | CTTGGTCTCAGCACTGTGAC |
| SD | TGTCCCTGACAACACTGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14084A-A9 | VGB6 | C57BL/6NTac | |
| 14084A-B9 | VGB6 | C57BL/6NTac | |
| 14084A-D1 | VGB6 | C57BL/6NTac | |
| 14084A-D2 | VGB6 | C57BL/6NTac | |
| 14084A-D6 | VGB6 | C57BL/6NTac | |
