| Gene | Mybbp1a |
| Chromosome | 11 |
| Deletion Start | 72,441,387 |
| Deletion End | 72,451,563 |
| Deletion Size | 10,177 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,441,474 | 72,441,494 | CGCGAGTTCTTGGACTTCTTC |
| TUP | 72,441,495 | 72,441,517 | TGGGACATTGCGAAACCGGATCA |
| TUR | 72,441,563 | 72,441,585 | TCCATCATACATTGGGCCTTGTG |
| TDF | 72,450,718 | 72,450,737 | TGGCTCCAATCGCCTCTATG |
| TDP | 72,450,740 | 72,450,765 | CTCTACTGGCAGGCCATGAGGATGCT |
| TDR | 72,450,786 | 72,450,804 | CCCAATGCTGGTCCCTCTC |
PCR Primers
| Primer | Sequence |
| SU | GTGGATTTGTTTGCACCTCC |
| SD | CACTTGCTGTGGCTTCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14089A-B9 | VGB6 | C57BL/6NTac | |
| 14089A-H7 | VGB6 | C57BL/6NTac | |
| 14089A-G8 | VGB6 | C57BL/6NTac | |
| 14089A-G4 | VGB6 | C57BL/6NTac | |
| 14089A-E9 | VGB6 | C57BL/6NTac | |
