| Gene | Ruvbl2 |
| Chromosome | 7 |
| Deletion Start | 45,434,260 |
| Deletion End | 45,421,893 |
| Deletion Size | 12,368 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,433,434 | 45,433,454 | GGAGTGACAGAGTCGTGGTTC |
| TUP | 45,433,405 | 45,433,431 | CCCACAATGCGTTCTGGAAACTTCGTC |
| TUR | 45,433,352 | 45,433,371 | GCAGGACACTGGGATGAGAC |
| TDF | 45,422,490 | 45,422,511 | TGAGGAGGAAGATGTGGAGATG |
| TDP | 45,422,465 | 45,422,487 | TGAGGACGCCTACACAGTGCTGA |
| TDR | 45,422,412 | 45,422,431 | CGCTGTGATGAGCTGGATGG |
PCR Primers
| Primer | Sequence |
| SU | TTTGGTGAGGAAGGTTGGAC |
| SD | GCAGGGACACTCAGAAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14090A-A10 | VGB6 | C57BL/6NTac | |
| 14090A-B11 | VGB6 | C57BL/6NTac | |
| 14090A-H5 | VGB6 | C57BL/6NTac | |
| 14090A-H7 | VGB6 | C57BL/6NTac | |
| 14090A-H12 | VGB6 | C57BL/6NTac | |
