| Gene | Shpk |
| Chromosome | 11 |
| Deletion Start | 73,199,522 |
| Deletion End | 73,223,205 |
| Deletion Size | 23,684 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,199,746 | 73,199,767 | CGCGAAGAAGTACCCGTGATTG |
| TUP | 73,199,769 | 73,199,791 | CCAGTCTGTCGGACCTCCAAGGC |
| TUR | 73,199,864 | 73,199,882 | GTGCCAGCAGGTGGGAAAG |
| TDF | 73,222,773 | 73,222,792 | TCTGACAGGTCTGGAGGTTG |
| TDP | 73,222,799 | 73,222,826 | CCACTGTGTATTCACGCATGATCCAGGC |
| TDR | 73,222,839 | 73,222,860 | GATGGTGAGATGGGTGTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | GAAGAGCATAGGCACCTTGG |
| SD | GCAGGTCTTCTCTGCTTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 137 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14091A-F7 | VGB6 | C57BL/6NTac | |
| 14091A-G4 | VGB6 | C57BL/6NTac | |
| 14091B-A4 | VGB6 | C57BL/6NTac | |
| 14091B-F4 | VGB6 | C57BL/6NTac | |
| 14091B-G4 | VGB6 | C57BL/6NTac | |
