| Gene | Ctdsp2 |
| Chromosome | 10 |
| Deletion Start | 126,995,903 |
| Deletion End | 126,996,531 |
| Deletion Size | 629 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,995,944 | 126,995,964 | CAAGGTACCTGACTGCAGGAG |
| TUP | 126,995,981 | 126,996,002 | AGAGCCAGCCCTCAGCCTAGGC |
| TUR | 126,996,009 | 126,996,033 | TCAGCATACTGGAAAAGAAGGAGAA |
| TDF | 126,996,255 | 126,996,276 | CGTGGGCTTTATCAGAGGAGTC |
| TDP | 126,996,279 | 126,996,300 | CAACCCCTGTGTGGCCCAGCAG |
| TDR | 126,996,305 | 126,996,326 | CCTTTCCTTTCCAGTGCCATTG |
PCR Primers
| Primer | Sequence |
| SU | GAAGGCTACTGTGGAGGCTG |
| SD | ACAGGTCTTGATGCTGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14093A-E5 | VGB6 | C57BL/6NTac | |
| 14093A-H8 | VGB6 | C57BL/6NTac | |
| 14093A-C8 | VGB6 | C57BL/6NTac | |
| 14093A-C3 | VGB6 | C57BL/6NTac | |
