| Gene | Tlr4 |
| Chromosome | 4 |
| Deletion Start | 66,827,835 |
| Deletion End | 66,841,546 |
| Deletion Size | 13,712 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,828,455 | 66,828,472 | TGGCTGCCAAGCCTGATG |
| TUP | 66,828,477 | 66,828,502 | TCTGAGTTCAAGCCTCAAGCCTCACA |
| TUR | 66,828,504 | 66,828,525 | GAGGAGATTCCCTGCCTCTAAC |
| TDF | 66,841,113 | 66,841,131 | AGCCATTGCTGCCAACATC |
| TDP | 66,841,133 | 66,841,156 | TCCAGGAAGGCTTCCACAAGAGCC |
| TDR | 66,841,185 | 66,841,206 | ACACCAACGGCTCTGAATAAAG |
PCR Primers
| Primer | Sequence |
| SU | GCAGTAAGCATCCTCTGTTG |
| SD | GGGAAAGTAAGAAACCGTTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14095B-C10 | VGB6 | C57BL/6NTac | |
| 14095B-D1 | VGB6 | C57BL/6NTac | |
| 14095B-E1 | VGB6 | C57BL/6NTac | |
| 14095B-E11 | VGB6 | C57BL/6NTac | |
| 14095B-H12 | VGB6 | C57BL/6NTac | |
