| Gene | Trpm8 |
| Chromosome | 1 |
| Deletion Start | 88,318,973 |
| Deletion End | 88,343,347 |
| Deletion Size | 24,375 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,319,775 | 88,319,794 | GGCTTCAGAGCCAGTCTATC |
| TUP | 88,319,798 | 88,319,823 | TGAGGCCACTTGGACTGAGTACTTCA |
| TUR | 88,319,844 | 88,319,862 | CCTGGAGGCCCTAGTCTTG |
| TDF | 88,343,227 | 88,343,249 | GTCCAGCCTTCAGCACTAATGAG |
| TDP | 88,343,250 | 88,343,277 | CAAGACAAGGACAACTGGAATGGACAGC |
| TDR | 88,343,301 | 88,343,320 | TCACTGGCAAGGTCCAACTG |
PCR Primers
| Primer | Sequence |
| SU | GACAGGGTTTGTCATAGGAC |
| SD | AAGAATGTGGCTGTCTTGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14096A-H3 | VGB6 | C57BL/6NTac | |
| 14096A-D4 | VGB6 | C57BL/6NTac | |
| 14096A-A12 | VGB6 | C57BL/6NTac | |
