| Gene | Sema3e |
| Chromosome | 5 |
| Deletion Start | 14,025,913 |
| Deletion End | 14,026,020 |
| Deletion Size | 108 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,025,922 | 14,025,942 | GGACACATCCTCACCTTGCTG |
| TUP | 14,025,951 | 14,025,973 | TCACCTGCTGGAACTCTGGACCC |
| TUR | 14,025,974 | 14,025,991 | GGTTCGCGGAGTGACCTG |
| TDF | 14,025,948 | 14,025,966 | GGGTCACCTGCTGGAACTC |
| TDP | 14,025,967 | 14,025,986 | TGGACCCCAGGTCACTCCGC |
| TDR | 14,025,987 | 14,026,005 | CCTGGGGTAGGAGGGGTTC |
PCR Primers
| Primer | Sequence |
| SU | CTGAAGCTAGCCACAGCCTC |
| SD | CAGTAGCTACAGCTCACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 58 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14098A-H7 | VGB6 | C57BL/6NTac | |
| 14098D-E10 | VGB6 | C57BL/6NTac | |
| 14098D-G7 | VGB6 | C57BL/6NTac | |
