| Gene | Txlnb |
| Chromosome | 10 |
| Deletion Start | 17,827,981 |
| Deletion End | 17,799,104 |
| Deletion Size | 28,878 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,799,240 | 17,799,259 | GAAGCCTGCATCCCGAGAAG |
| TUP | 17,799,264 | 17,799,289 | CCCATGATGTCGCGGAAGAGTTGAGC |
| TUR | 17,799,316 | 17,799,335 | GACTGGCAGCAGACCCATAC |
| TDF | 17,827,717 | 17,827,739 | CAGTGACCTAATCCTGGGTTCTC |
| TDP | 17,827,741 | 17,827,768 | AAACTATGGGTACCTCTAAACCCGCACA |
| TDR | 17,827,789 | 17,827,809 | CCGTTGGAGGGTTTCTTCCTG |
PCR Primers
| Primer | Sequence |
| SU | GAAAATGTCTATGTGCAAGCAC |
| SD | AGGACTGTGCAGGATGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14099A-B5 | VGB6 | C57BL/6NTac | |
| 14099B-C8 | VGB6 | C57BL/6NTac | |
| 14099C-C3 | VGB6 | C57BL/6NTac | |
