| Gene | 4632428N05Rik |
| Chromosome | 10 |
| Deletion Start | 60,368,984 |
| Deletion End | 60,347,025 |
| Deletion Size | 21,960 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,347,252 | 60,347,274 | CAGTGGTGTCTCCATCCTTCATC |
| TUP | 60,347,275 | 60,347,304 | AGGCACATCTTTAATTGCTAACAGCTGCCA |
| TUR | 60,347,344 | 60,347,361 | TCGGGTCAGGGAAGTGTC |
| TDF | 60,368,473 | 60,368,491 | TCTGGGTGGCACTGATCTC |
| TDP | 60,368,492 | 60,368,515 | CAGGTGGCACTGATCGCTCCCAAT |
| TDR | 60,368,523 | 60,368,543 | CCTTGGGTGTTGCTGCTAGAG |
PCR Primers
| Primer | Sequence |
| SU | CAAGGCAAGCTGGGTTAGAC |
| SD | CATGAAGAAAGGCTCTTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14100A-A10 | VGB6 | C57BL/6NTac | |
| 14100A-B7 | VGB6 | C57BL/6NTac | |
| 14100A-E8 | VGB6 | C57BL/6NTac | |
| 14100A-A9 | VGB6 | C57BL/6NTac | |
| 14100A-H1 | VGB6 | C57BL/6NTac | |
| 14100A-G5 | VGB6 | C57BL/6NTac | |
