| Gene | Tnfrsf19 |
| Chromosome | 14 |
| Deletion Start | 61,024,809 |
| Deletion End | 60,996,610 |
| Deletion Size | 28,200 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,024,786 | 61,024,809 | GCACTCAAGGTCCTACCTCTACAC |
| TUP | 61,024,764 | 61,024,785 | AGGACGGTGCTCTTCGCTGCCA |
| TUR | 61,024,722 | 61,024,746 | TGAAAGAGAAGAGGCAACTTACCAG |
| TDF | 60,996,798 | 60,996,819 | GCCTTTGGCTGCTTCTTACTTC |
| TDP | 60,996,772 | 60,996,796 | CCCAGAACTGGTTCTAGAGGCTGCC |
| TDR | 60,996,752 | 60,996,770 | GCGTCAGGTCAAGGGTCAC |
PCR Primers
| Primer | Sequence |
| SU | AAATAACATTTAGCGCCTCG |
| SD | CCCTGTGTGGTCCTATCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14102C-C4 | VGB6 | C57BL/6NTac | |
| 14102C-G1 | VGB6 | C57BL/6NTac | |
| 14102B-B2 | VGB6 | C57BL/6NTac | |
| 14102B-F3 | VGB6 | C57BL/6NTac | |
