| Gene | Vps52 |
| Chromosome | 17 |
| Deletion Start | 33,966,193 |
| Deletion End | 33,956,096 |
| Deletion Size | 10,098 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,956,237 | 33,956,258 | CAGGCGTAGGCTCCATTTAGAC |
| TUP | 33,956,259 | 33,956,283 | AAGAGGTTCGGAGCCGTCTTGCCAC |
| TUR | 33,956,314 | 33,956,334 | GCTTGGGTTTCAGCCACTTTG |
| TDF | 33,965,676 | 33,965,697 | TGATCCGTGGCTTTGGTAGTTC |
| TDP | 33,965,702 | 33,965,727 | AAGGCATCAGTGGAGTCCCTGAGTCA |
| TDR | 33,965,749 | 33,965,770 | GCTGGTTCCATTTCGGAAGTTG |
PCR Primers
| Primer | Sequence |
| SU | GAGACAGTTTTGGAAGGGAC |
| SD | GGAGAATTTAGAGGCCCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14109A-A11 | VGB6 | C57BL/6NTac | |
| 14109A-B7 | VGB6 | C57BL/6NTac | |
| 14109A-B10 | VGB6 | C57BL/6NTac | |
| 14109A-E5 | VGB6 | C57BL/6NTac | |
| 14109A-H1 | VGB6 | C57BL/6NTac | |
| 14109A-F12 | VGB6 | C57BL/6NTac | |
