| Gene | Dusp5 |
| Chromosome | 19 |
| Deletion Start | 53,541,421 |
| Deletion End | 53,529,321 |
| Deletion Size | 12,101 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,529,789 | 53,529,809 | GGAACTCTGGACCGGAGAAAG |
| TUP | 53,529,810 | 53,529,833 | TCACCACTTGCGGGAGTATGCACC |
| TUR | 53,529,839 | 53,529,856 | CCCAAGCGCACGTCAAAC |
| TDF | 53,540,904 | 53,540,925 | GCCTCTTTCCAGACTGTGTCAG |
| TDP | 53,540,928 | 53,540,952 | AAGAAGGAGGCAAGGTCCTGGTCCA |
| TDR | 53,541,004 | 53,541,024 | AGCCGGAACTGCTTGGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CCACTTCCTCTTTCTCGCTC |
| SD | TAGGTGAATTGGCTTCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 121 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14112A-A10 | VGB6 | C57BL/6NTac | |
| 14112A-E10 | VGB6 | C57BL/6NTac | |
| 14112A-F6 | VGB6 | C57BL/6NTac | |
