| Gene | Ptpn6 |
| Chromosome | 6 |
| Deletion Start | 124,732,950 |
| Deletion End | 124,721,031 |
| Deletion Size | 11,920 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,732,663 | 124,732,681 | GGTTCCCTGGGTTGTGTTG |
| TUP | 124,732,626 | 124,732,654 | TGATACATTTCTTCCCTTCTGTCCCTGCC |
| TUR | 124,732,579 | 124,732,601 | AGGCATGGAGTAGACATAGACAG |
| TDF | 124,721,640 | 124,721,660 | AGGGCCAGGAGTCGGAGTATG |
| TDP | 124,721,611 | 124,721,635 | TATCACGTACCCTCCCGCTGTGAGG |
| TDR | 124,721,580 | 124,721,600 | AGGAAGTACGCGAGGCTTTGG |
PCR Primers
| Primer | Sequence |
| SU | TGACTCTTGACCTGCCACAC |
| SD | TTGGAGGCAATACACAGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14114A-A9 | VGB6 | C57BL/6NTac | |
| 14114A-B1 | VGB6 | C57BL/6NTac | |
| 14114A-C1 | VGB6 | C57BL/6NTac | |
| 14114A-E9 | VGB6 | C57BL/6NTac | |
