| Gene | Msmp |
| Chromosome | 4 |
| Deletion Start | 43,584,265 |
| Deletion End | 43,583,261 |
| Deletion Size | 1,005 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,583,893 | 43,583,916 | TGCCACTACGAGGGTAAATACTTC |
| TUP | 43,583,868 | 43,583,892 | ACTCTGGGTGAATCTTGGCTCCGCA |
| TUR | 43,583,818 | 43,583,837 | ACAGCAGCCCACACCAACAG |
| TDF | 43,583,507 | 43,583,525 | ACTGTCCTGACTCCCAACC |
| TDP | 43,583,481 | 43,583,504 | TGGTTCTCCAGGGCTCAGCATCCT |
| TDR | 43,583,444 | 43,583,465 | CCTCCTGTAGCACTTCACACTC |
PCR Primers
| Primer | Sequence |
| SU | CCTCTCTGCAGGTCAAGGAG |
| SD | CCAGGATTGGTACTTCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14137A-D5 | VGB6 | C57BL/6NTac | |
| 14137A-H6 | VGB6 | C57BL/6NTac | |
