| Gene | Zscan30 |
| Chromosome | 18 |
| Deletion Start | 23,971,786 |
| Deletion End | 23,971,067 |
| Deletion Size | 720 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TGCTGGTGCTAGAGCAGTTC |
| TUP | | | CATCTTGCCTATGGAGCTGAAGGCC |
| TUR | | | CACTTCTGGGCCTGTGTTCTTG |
| TDF | | | GTCATCTGACAGTTTTCTGCTTC |
| TDP | | | TCTGTCTTGCCTTGTTGCTGTAGGGT |
| TDR | | | TGTGACTGAGTGCAGCTAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CTGTGGTCACTGAGCTGCAT |
| SD | CCTTGGCACATTCGGTAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14178A-H5 | VGB6 | C57BL/6NTac | |
| 14178A-B3 | VGB6 | C57BL/6NTac | |
| 14178A-D4 | VGB6 | C57BL/6NTac | |
