| Gene | Tomt |
| Chromosome | 7 |
| Deletion Start | 101,902,268 |
| Deletion End | 101,900,265 |
| Deletion Size | 2,004 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,902,115 | 101,902,135 | AGGAGCGGGCTTTCAGCTATG |
| TUP | 101,902,091 | 101,902,111 | TCACCCATGCCCTGCCTGGAG |
| TUR | 101,902,063 | 101,902,082 | TCAAGCGTGGTGAGGATGTG |
| TDF | 101,900,631 | 101,900,651 | TCACCTCCAGCTATGGCTTTC |
| TDP | 101,900,602 | 101,900,627 | ACAGGTGGAGCTCATTGCAGGCAGCT |
| TDR | 101,900,576 | 101,900,596 | TCTTAGGCGTGGGATCACTTC |
PCR Primers
| Primer | Sequence |
| SU | GATCAGAGGAGTCCAGGCAG |
| SD | TTCAGCCAGCTGTCTCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14190A-G7 | VGB6 | C57BL/6NTac | |
| 14190A-B9 | VGB6 | C57BL/6NTac | |
| 14190A-C1 | VGB6 | C57BL/6NTac | |
| 14190A-C2 | VGB6 | C57BL/6NTac | |
| 14190A-E11 | VGB6 | C57BL/6NTac | |
