| Gene | Stxbp3b |
| Chromosome | 19 |
| Deletion Start | 9,559,180 |
| Deletion End | 9,557,208 |
| Deletion Size | 1,973 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 9,558,453 | 9,558,471 | GCCGGAAAGAAGGCGAATG |
| TUP | 9,558,401 | 9,558,429 | TTACCACCAAACTTTTGTCGTCATGCTGC |
| TUR | 9,558,355 | 9,558,382 | TATTCTCTATAACAGTTATGCCCTCCTC |
| TDF | 9,558,102 | 9,558,128 | CATTCCACAGGAATCTCAGGTTTATAC |
| TDP | 9,558,046 | 9,558,075 | ACTGTTACAGTCCAGACCCTAGTAATGCCA |
| TDR | 9,557,936 | 9,557,963 | GGCATTATCTAGAGGTTTACTCTTGTAC |
PCR Primers
| Primer | Sequence |
| SU | GAGCCTCCCCACCGCGA |
| SD | CAAACTTAGAGAACGCCTAGA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 193 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14194A-F7 | VGB6 | C57BL/6NTac | |
