| Gene | Ryr3 |
| Chromosome | 2 |
| Deletion Start | 113,030,328 |
| Deletion End | 113,028,580 |
| Deletion Size | 1,749 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,029,687 | 113,029,709 | TGTCTTTCTAGCACCTGGTTGAC |
| TUP | 113,029,659 | 113,029,685 | TGAAGGCAGGATCAAAAGGCAAAGGGA |
| TUR | 113,029,639 | 113,029,657 | AGCCACCACCTTGCCAATG |
| TDF | 113,028,648 | 113,028,669 | TGCAAACATTCACAAGGAGCAG |
| TDP | 113,028,626 | 113,028,647 | CGGAAGTTCTGCCTGGCAGCCG |
| TDR | 113,028,602 | 113,028,622 | AGCACAGGCGATTCCCAAGTC |
PCR Primers
| Primer | Sequence |
| SU | TAGGCATGCTCTTGGGTAGG |
| SD | AGGAATCTCTGGTATACTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14213A-C1 | VGB6 | C57BL/6NTac | |
| 14213A-B3 | VGB6 | C57BL/6NTac | |
| 14213A-F5 | VGB6 | C57BL/6NTac | |
