| Gene | Plcl1 |
| Chromosome | 1 |
| Deletion Start | 55,695,754 |
| Deletion End | 55,715,597 |
| Deletion Size | 19,844 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CAGTGCCCACGACTGCATTAG |
| TUP | | | CTTCATGCAGGCTGGCTGCGA |
| TUR | | | TCGGGCGCACTTTCTTCAG |
| TDF | | | CACACTCTCCCGAGCTTACC |
| TDP | | | TGACCTCCCATAGCCTTCTACATAGAATCC |
| TDR | | | CAAGGAGCAAAGCCTCCAATG |
PCR Primers
| Primer | Sequence |
| SU | GCTTTATTCTGGGAGGACAC |
| SD | AGTTCTGTCCAGGCTTTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14220A-C2 | VGB6 | C57BL/6NTac | |
