14253   Gtf3a     Status: ES cell colonies screened / QC positives

GeneGtf3a
Chromosome5
Deletion Start146,953,259
Deletion End146,955,540
Deletion Size2,282
Genome Build38
MGI IDMGI:1913846
NCBI Gene ID66596
BAC ID292G8
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF146,953,592146,953,614ACTGTGTCAGAGGGTAAAGGTAG
TUP146,953,616146,953,641TGCTATGAGGGCATACTTCCTCGTGC
TUR146,953,643146,953,663GCGTCCTCGGTGTGATAACTG
TDF146,955,025146,955,042GGGAACCAAACCCGTGTC
TDP146,955,044146,955,068TCCTCGAGCAGCGAATGCTGTAAGC
TDR146,955,103146,955,122TGCAGTGCAAGGGAAAGAAG

PCR Primers

PrimerSequence
SUCACGAGGGTACGTAGATGGG
SDCAGCTCTGAGTCCTGTCGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR72 bp++-
TDFTDR98 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14253A-A1VGB6C57BL/6NTac
14253A-B12VGB6C57BL/6NTac
14253A-D6VGB6C57BL/6NTac
14253A-F1VGB6C57BL/6NTac
14253A-F11VGB6C57BL/6NTac