| Gene | Gtf3a |
| Chromosome | 5 |
| Deletion Start | 146,953,259 |
| Deletion End | 146,955,540 |
| Deletion Size | 2,282 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 146,953,592 | 146,953,614 | ACTGTGTCAGAGGGTAAAGGTAG |
| TUP | 146,953,616 | 146,953,641 | TGCTATGAGGGCATACTTCCTCGTGC |
| TUR | 146,953,643 | 146,953,663 | GCGTCCTCGGTGTGATAACTG |
| TDF | 146,955,025 | 146,955,042 | GGGAACCAAACCCGTGTC |
| TDP | 146,955,044 | 146,955,068 | TCCTCGAGCAGCGAATGCTGTAAGC |
| TDR | 146,955,103 | 146,955,122 | TGCAGTGCAAGGGAAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CACGAGGGTACGTAGATGGG |
| SD | CAGCTCTGAGTCCTGTCGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14253A-A1 | VGB6 | C57BL/6NTac | |
| 14253A-B12 | VGB6 | C57BL/6NTac | |
| 14253A-D6 | VGB6 | C57BL/6NTac | |
| 14253A-F1 | VGB6 | C57BL/6NTac | |
| 14253A-F11 | VGB6 | C57BL/6NTac | |
