| Gene | Gm1968 |
| Chromosome | 16 |
| Deletion Start | 29,962,304 |
| Deletion End | 29,958,365 |
| Deletion Size | 3,940 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,962,248 | 29,962,267 | GGTTCCTGATGGGCAAACAG |
| TUP | 29,962,219 | 29,962,245 | TGACCAGTTGGTTGCAGCACTAAGGAA |
| TUR | 29,962,181 | 29,962,205 | GCCATTCAGCTAAAGACCAAGAAAG |
| TDF | 29,959,171 | 29,959,190 | ACCACAAGGCCCACGAACTC |
| TDP | 29,959,141 | 29,959,168 | TTGGCGTATGACTACAGCATCCTAAGCA |
| TDR | 29,959,084 | 29,959,102 | CTCAGGCTCAGGGAGGAAC |
PCR Primers
| Primer | Sequence |
| SU | GCTTACAGGGAGATTCTTGGG |
| SD | CTGTAGCACAGGATATCAGTA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14259A-A6 | VGB6 | C57BL/6NTac | |
| 14259A-B1 | VGB6 | C57BL/6NTac | |
| 14259A-E8 | VGB6 | C57BL/6NTac | |
| 14259A-G11 | VGB6 | C57BL/6NTac | |
| 14259A-H4 | VGB6 | C57BL/6NTac | |
