| Gene | Ctps2 |
| Chromosome | X |
| Deletion Start | 162,903,476 |
| Deletion End | 162,934,371 |
| Deletion Size | 30,896 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 162,903,498 | 162,903,521 | GGGTCATCTCAGGCATTGGTAAAG |
| TUP | 162,903,527 | 162,903,551 | ATCGCCAGCAGCATTGGGACTATTC |
| TUR | 162,903,553 | 162,903,574 | AACTCGGAGTCCACATGACTTG |
| TDF | 162,933,838 | 162,933,859 | TGGAACTCAGGGTCTTGCATAG |
| TDP | 162,933,862 | 162,933,881 | TTTGCCACTGAGTTATACCC |
| TDR | 162,933,928 | 162,933,951 | CAGGAAAGTGGAAGAGGGAGATTC |
PCR Primers
| Primer | Sequence |
| SU | GTCTCTTGCATGGTCTGCAG |
| SD | TCTTTCCCTCTAATGCAGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14281A-D9 | VGB6 | C57BL/6NTac | |
| 14281A-E6 | VGB6 | C57BL/6NTac | |
| 14281A-G9 | VGB6 | C57BL/6NTac | |
