| Gene | Cnga2 |
| Chromosome | X |
| Deletion Start | 72,003,296 |
| Deletion End | 72,009,525 |
| Deletion Size | 6,230 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,004,021 | 72,004,041 | GGTGGCTTCCGAAGGTAAGTC |
| TUP | 72,004,053 | 72,004,081 | CTGACCTGCTACCTCTTCTGTTCTTGGGA |
| TUR | 72,004,085 | 72,004,107 | GGAGGGCCTAGTATCCATTTGAC |
| TDF | 72,008,596 | 72,008,619 | TCCTGATTGGTGTCCTCATCTTTG |
| TDP | 72,008,620 | 72,008,646 | CCACTATTGTGGGAAATGTGGGCTCCA |
| TDR | 72,008,654 | 72,008,674 | GCTCGTGTGGCATTCATGTTG |
PCR Primers
| Primer | Sequence |
| SU | CCCATTCTTCCTGACTGTGG |
| SD | TTGTGGTGCACAGTGTTCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14283A-E12 | VGB6 | C57BL/6NTac | |
| 14283A-G5 | VGB6 | C57BL/6NTac | |
| 14283B-D11 | VGB6 | C57BL/6NTac | |
