| Gene | Cdx1 |
| Chromosome | 18 |
| Deletion Start | 61,036,117 |
| Deletion End | 61,019,700 |
| Deletion Size | 16,418 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,035,398 | 61,035,417 | GGCAAGGCCACAAGTAGAAG |
| TUP | 61,035,375 | 61,035,396 | CCAGATGCCCAGTCGTCGCAGC |
| TUR | 61,035,326 | 61,035,347 | GCAGGGTTAGGAGTGGAAGAAG |
| TDF | 61,020,528 | 61,020,549 | CCCTTCCCTCATTGCCCTTCTG |
| TDP | 61,020,501 | 61,020,526 | TCCTCAGGTAAGACCCGAACCAAGGA |
| TDR | 61,020,466 | 61,020,485 | GGCGTTGGTGGTCTGTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTCTCCACCTGTAACCCAGG |
| SD | CTCGTTGAGCCCTCTGAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14287A-E9 | VGB6 | C57BL/6NTac | |
| 14287A-B4 | VGB6 | C57BL/6NTac | |
| 14287A-C11 | VGB6 | C57BL/6NTac | |
| 14287A-A10 | VGB6 | C57BL/6NTac | |
